Calotropis gigantea Assisted Synthesis of Zinc Oxide Nanoparticle Catalysis: Synthesis of Novel 3-Amino Thymoquinone Connected 1,4-Dihyropyridine Derivatives and Their Cytotoxic Activity

The synthesis of biologically active 1,4-dihyripyridine derivatives using a Calotropis gigantea leaf powder and ZnO NPs used as catalyst under solvent-free conditions at room temperature via grinding method has been established in single-stage, mild, environmentally friendly green process. procedure is fast effective produces high yields. Three cancer cell lines were to assess the cytotoxic activity 1,4-dihyropyridine derivatives. cytotoxicity compound 1f (HepG2, LC50-0.50 μM, MCF-7, LC50-0.64 HeLa, LC50-0.52 μM) was found be highly active. synthesized demonstrated their safety by causing substantially less normal HEK-293, LO2, MRC5 with IC 50 > 100 g/mL. As result, could serve high-impact molecule for production anticancer drugs future.